tinroИзвестия ТИНРОIzvestiya TINRO1606-99192658-5510ТИНРО10.26428/1606-9919-2015-182-269-276tinro-285Research ArticleТЕХНОЛОГИЯ ОБРАБОТКИ ГИДРОБИОНТОВTECHNOLOGY FOR PROCESSING OF AQUATIC ORGANISMS,Антирадикальный эффект низкомолекулярных пептидов экстрактов и гидролизатов тканей гидробионтовAntiradical effect of low-molecular peptides in extracts and hydrolyzates from tissues of water organismsКарауловаЕкатерина ПавловнаKaraulovaEkaterina P.karaulova@tinro.ruЧепкасоваАнна ИвановнаChepkasovaAnna I.chepkasova@mail.ruСлуцкаяТатьяна НоевнаSlutskayaTatiana N.slutskaya@tinro.ruШульгинаЛидия ВасильевнаShulginaLidia V.lvshulgina@tinro.ruЯкушЕвгений ВалентиновичYakushEugeny V.evyakush@mail.ruТихоокеанский научно-исследовательский рыбохозяйственный центрRussian Federation2015300920151823269276Copyright © Караулова Е.П., Чепкасова А.И., Слуцкая Т.Н., Шульгина Л.В., Якуш Е.В., 20152015Караулова Е.П., Чепкасова А.И., Слуцкая Т.Н., Шульгина Л.В., Якуш Е.В.Karaulova E.P., Chepkasova A.I., Slutskaya T.N., Shulgina L.V., Yakush E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://izvestiya.tinro-center.ru/jour/article/view/285

Исследован молекулярно-массовый состав пептидов экстрактов и гидролизатов мягких тканей корбикулы японской, мерценарии и печени кеты. Определена антирадикальная активность в экстрактах и гидролизатах мягких тканей корбикулы японской, мерценарии и печени кеты. Обнаружена корреляция между содержанием пептидной фракции с молекулярной массой 3-4 кДа и величиной антирадикальной активности. В экстрактах антирадикальной активностью обладают низкомолекулярные пептиды, высокомолекулярные белки и свободные аминокислоты. Отмечено увеличение антирадикальной активности в гидролизатах по сравнению с экстрактами. Установлено, что в гидролизатах антирадикальной активностью обладают только пептидные фракции с молекулярной массой 2,5-4,5 кДа.

Molecular-weight composition of peptides in extracts and hydrolyzates from soft tissues of the clams Corbicula japonica and Mercenaria mercenaria and liver of chum salmon is investigated. Antiradical activity is defined for low-molecular peptides, as well as for high-molecular proteins and free amino acids in the extracts. The maximum activity is detected for the extracts of salmon liver; all protein fractions in the water extract of the liver have antiradical activity. Low-molecular peptides and free amino acids have antiradical activity in all samples. The peptides with molecular weight 4.3 kDa have the highest activity in the water extract from C. japonica (43 units) and the peptides with molecular weight 4.7 kDa - in the water extract from M. mercenaria (5.6 units). After hydrolysis, the portion of low-molecular peptides increases for C. japonica , M. mercenaria , and salmon liver in 22.1, 14.5, and 11.1 %, respectively. Hence antiradical activity for hydrolyzates from C. japonica and M. mercen aria is in 1.9 times higher and for hydrolyzates from salmon liver - in 1.3 times higher than for water extracts from their tissues. Only the peptides with molecular weight 2.8-4.7 kDa have antiradical activity in the hydrolyzates; its value is 1-24 activity units for M. mercenaria ; 13-76 units for C. japonica and about 40 units for the chum salmon liver. Correlation is found between the content of peptides with molecular weight 3-4 kDa in hydrolyzates and their antiradical activity.

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The authors declare that there are no conflicts of interest present.