tinroИзвестия ТИНРОIzvestiya TINRO1606-99192658-5510ТИНРО10.26428/1606-9919-2018-195-253-264tinro-437Research ArticleТЕХНОЛОГИЯ ОБРАБОТКИ ГИДРОБИОНТОВTECHNOLOGY FOR PROCESSING OF AQUATIC ORGANISMS,ИССЛЕДОВАНИЕ БИОЛОГИЧЕСКОЙ АКТИВНОСТИ ТКАНЕЙ ДВУСТВОРЧАТЫХ МОЛЛЮСКОВStudy on biological activity of tissues from bivalve mollusksКарауловаЕ. П.KaraulovaE. P.

Караулова Екатерина Павловна, кандидат технических наук, старший научный сотрудник

690091, г. Владивосток, пер. Шевченко, 4

Karaulova Ekaterina P., Ph.D., senior researcher

karaulova2002@yandex.ruЮнХ. д.YoonH. D.

Юн Ху Донг, доктор философии, директор

120-12, г. Косон

Yoon Ho Dong, Ph.D., director

hdyyoon@gmail.comКимДж. Г.KimJ. G.

Ким Джонг Гун, профессор

38, г. Тхоньян

Kim Jeong Gyun, professor

kimjeonggyun@nate.comПакС. Х.ParkS. H.

Пак Си Хан, доктор философии, заведующая отделом

38, г. Тхоньян

Park Si Hyang, Ph.D., head of department

hyangi51@hanmail.comСлуцкаяТ. Н.SlutskayaT. N.

Слуцкая Татьяна Ноевна, доктор технических наук, профессор, заведующая отделом

690091, г. Владивосток, пер. Шевченко, 4

Slutskaya Tatiana N., D.Sc., professor, head of department

tinro@tinro-center.ruЯкушЕ. В.YakushE. V.

Якуш Евгений Валентинович, кандидат химических наук, доцент, заместитель директора

690091, г. Владивосток, пер. Шевченко, 4

Yakush Eugeny V., Ph.D., deputy director

evyakush@mail.ruТихоокеанский научно-исследовательский рыбохозяйственный центрRussian FederationБиологическая пищевая компанияKorea, Republic ofКенгсанский национальный университетKorea, Republic of2018301220181954253264Copyright © Караулова Е.П., Юн Х.д., Ким Д.Г., Пак С.Х., Слуцкая Т.Н., Якуш Е.В., 20182018Караулова Е.П., Юн Х.д., Ким Д.Г., Пак С.Х., Слуцкая Т.Н., Якуш Е.В.Karaulova E.P., Yoon H.D., Kim J.G., Park S.H., Slutskaya T.N., Yakush E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://izvestiya.tinro-center.ru/jour/article/view/437

Исследована биологическая активность тканей и ферментных гидролизатов трех видов двустворчатых моллюсков: корбикула японская Corbicula japonica, мерценария Mercenaria mercenaria, анадара Броутона Anadara broughtonii. Установлено, что при ферментативном гидролизе исследуемых мягких тканей двустворчатых моллюсков, происходит достоверное увеличение величины антирадикальной активности для всех исследуемых образцов: в 8,0 раза для C. japonica, в 3,0 раза для M. mercenaria и в 2,5 раза для A. broughtonii. Максимальная антитромбоцитарная активность была определена для гидролизата мягких тканей C. japonica при концентрации белкового гидролизата 10 мг/мл и составила 0,0823 ед. Ангиотензин-ингибирующей активностью обладали только гидролизаты M. mercenaria в концентрации гидролизата 10–100 мг/мл. Максимальная активность в отношении альфа-амилазы и альфа-гликозидазы наблюдалась для гидролизатов мягких тканей M. mercenaria и составила соответственно 7,5 и 7,2 % при концентрации гидролизата 1 мкг/мл. Гепатопротекторная активность, измеренная по выживаемости гепатоцитов HepG2, проявлялась при концентрации гидролизата корбикулы и мерценарии от 100 мкг/мл, анадары — от 200 мкг/мл.

Biological activity of tissues and enzyme hydrolyzates is investigated for three species of bivalve mollusks: Corbicula japonica, Mercenaria mercenaria, and Anadara broughtonii. Antiradical activity of all samples has increased signifcantly during their enzymatic hydrolysis: in eight times for C. japonica, in three times for M. mercenaria, and in 2.5 times for A. broughtonii. The highest anti-trombolitic activity (0.0823 units) is found for the hydrolyzate of C. japonica in concentration of 10 mg/mL. Angiotensin I-converting enzyme inhibitory activity is found only for the hydrolyzate of M. mercenaria in concentration of 10–100 mg/mL. The maximum inhibitory activity for alpha-amylase and alpha-glycosidase (7.5 and 7.2 %, respectively) is observed for the hydrolyzate of M. mercenaria in concentration 1 mg/mL. Hepatoprotective activity, measured by survival rate of HepG2 hepatocytes, is detected for the hydrolyzate of tissues for all species in weak concentrations: 0.1 mg/mL for C. japonica and M. mercenaria and 0.2 mg/mL for A. broughtonii.

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The authors declare that there are no conflicts of interest present.