tinroИзвестия ТИНРОIzvestiya TINRO1606-99192658-5510ТИНРО10.26428/1606-9919-2019-196-63-71tinro-447Research ArticleБИОЛОГИЧЕСКИЕ РЕСУРСЫBIOLOGICAL RESOURCESВОЗДЕЙСТВИЕ ЭКСТРЕМАЛЬНОЙ СОЛЕНОСТИ ВОДЫ НА МОРСКОГО КАРАСЯ SPARIDENTEX HASTA: ИССЛЕДОВАНИЕ ХЛОРИДНЫХ КЛЕТОК В ЖАБРАХ И УРОВНЯ ГЛЮКОЗЫ В ПЛАЗМЕ КРОВИEXPOSURE OF SPARIDENTEX HASTA TO SALINITY CHALLENGE: A STUDY OF GILL CHLORIDE CELLS AND PLASMA GLUCOSE LEVELМезеноваО. Я.MezenovaO. Ya.

Мезенова Ольга Яковлевна, доктор технических наук, профессор

Mezenova Olga Yakovlevna, Doctor of Technical Sciences, Professor of the Department of Food Biotechnology

mezenova@klgtu.ruМоровватиХ.MorovvatiH.

Мороввати Хасан, доктор биологических наук, профессор

Morovvati Hassan, Doctor of Biological Sciences, Professor of Department of Histology & Anatomy

hmorovvati@ut.ac.irКешткарС.KeshtkarS.

Кешткар Саид, аспирант

Keshtkar Saeid, Ph.D student of Food Biotechnology Departrment

keshtkar.bio@gmail.comРомианиЭ.RomianiE.

Ромиани Эхсан, аспирант

Roomiani Ehsan, Ph.D student of Comparative Histology Department

eroomiani@ut.ac.irKaliningrad State Technical UniversityRussian FederationTehran UniversityIslamic Republic of Iran20193003201919616371Copyright © Мезенова О.Я., Мороввати Х., Кешткар С., Ромиани Э., 20192019Мезенова О.Я., Мороввати Х., Кешткар С., Ромиани Э.Mezenova O.Y., Morovvati H., Keshtkar S., Romiani E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://izvestiya.tinro-center.ru/jour/article/view/447

Рассмотрено влияние различной солености воды на количество и площадь хлоридных клеток в жабрах, а также на уровень глюкозы в плазме крови морского карася Sparidentex hasta. Для этой цели 180 рыб со средней длиной 20–25 см и средней массой 200 г содержались на Исследовательской станции им. Имама Хомейни в течение 14 сут в воде с различной соленостью: 5, 20, 40 и 60 ppt. Эксперимент с соленостью воды 40 ppt считался контрольным. В течение эксперимента рыбу кормили коммерческим кормом фирмы Biomar. Отбор проб крови рыбы проводили соответственно через 6 ч, 12 ч, 1 сут, 2, 7 и 14 сут после нахождения в воде с заданным уровнем солености. Исследование жабр и крови осуществляли в лаборатории биотехнологии Университета морской науки и технологии (г. Хоррамшахр, Иран). Измерения уровня глюкозы в плазме (мг/100 мл) проводили колориметрическим методом анализатором Technicon RA1000 (США) с использованием коммерческого набора MAN. Образцы хлоридных клеток в жабрах рыб подвергали морфологическим и гистометрическим исследованиям, отмечая особенности клеток, изменение их площади после воздействия воды различной солености, данные сравнивались с данными контрольных образцов. Проанализированы литературные данные, в которых отмечены аналогичные зависимости у других видов рыб. Результаты исследования показали изменения уровня глюкозы, количества и площади хлоридных клеток в жабрах Sparidentex hasta с течением времени и под действием различной солености воды. Установлено, что Sparidentex hasta обладает высокой способностью переносить изменения солености в окружающей среде, что обусловлено физиологическими механизмами осморегуляции и особенностями вовлеченных органов, прежде всего жабр.

The effects of different water salinities on the number and area of chloride (mitochondriarich) cells and on plasma glucose level in Sparidentex hasta were studied. Fish were exposed to a salinity of 5, 20, 40, and 60 ppt for 14 days. The group kept at 40 ppt was considered as the control. Samples were collected at 6 and 12 h and on days 1, 2, 7, and 14 of exposure. The results of this study revealed changes in the plasma glucose level and in the number and area of chloride cells in S. hasta associated with water salinity variations. It can be concluded that S. hasta has a wide tolerance towards salinity changes in the environment, which is provided by the mechanisms of osmoregulation and the involved organs, mostly by gills.

Sparidentex hastacоленость водыхлоридные (митохондриальные) клеткисодержание глюкозы в кровиадаптация к экстремальным условиямSparidentex hastawater salinitychloride cells/mitochondria-rich cellsplasma glucose levelextreme habitat adaptationThis research was partially supported by the Fisheries Research Institute of Khuzestan Province Fisheries Department. We would like to thank professor Sawari for his expert advice and encouragement throughout this project and also our dear colleagues, engineers and laboratory assistants, as well as Reza Sahraeian, Nima Yazdi and Mohsen Heydari who greatly assisted the research.ReferencesAltinok, I., Galli, S.M., and Chapman, F.A., Ionic and osmotic regulation capabilities of juvenile Gulf of Mexico sturgeon, Acipenser oxyrinchus de sotoi, Comp. Biochem. 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The authors declare that there are no conflicts of interest present.