tinroИзвестия ТИНРОIzvestiya TINRO1606-99192658-5510ТИНРО10.26428/1606-9919-2022-202-692-705tinro-763Research ArticleТЕХНОЛОГИЯ ОБРАБОТКИ ГИДРОБИОНТОВTECHNOLOGY FOR PROCESSING OF AQUATIC ORGANISMS,Антирадикальные свойства пептидов гидробионтовAntiradical properties of peptides from hydrobiontshttps://orcid.org/0000-0001-8508-9603КарауловаЕ. П.KaraulovaE. P.

Караулова Екатерина Павловна, кандидат технических наук, ведущий научный сотрудник

690091, г. Владивосток, пер. Шевченко, 4

Ekaterina P. Karaulova, Ph.D., senior researcher

4, Shevchenko Alley, Vladivostok, 690091

karaulova2002@yandex.ruhttps://orcid.org/0000-0002-3228-3047СлуцкаяТ. Н.SlutskayaT. N.

Слуцкая Татьяна Ноевна, доктор технических наук, профессор

690091, г. Владивосток, пер. Шевченко, 4

Tatiana N. Slutskaya, D.Tech., professor

4, Shevchenko Alley, Vladivostok, 690091

tatyana.slutskaya@tinro-center.ruhttps://orcid.org/0000-0001-7837-5943ЯкушЕ. В.YakushE. V.

Якуш Евгений Валентинович, кандидат химических наук, первый заместитель руководителя филиала

690091, г. Владивосток, пер. Шевченко, 4

Eugeny V. Yakush, Ph.D., deputy director

4, Shevchenko Alley, Vladivostok, 690091

evyakush@mail.ruТихоокеанский филиал ВНИРО (ТИНРО)РоссияPacific branch of VNIRO (TINRO)Russian Federation2022051020222023692705Copyright © Караулова Е.П., Слуцкая Т.Н., Якуш Е.В., 20222022Караулова Е.П., Слуцкая Т.Н., Якуш Е.В.Karaulova E.P., Slutskaya T.N., Yakush E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://izvestiya.tinro-center.ru/jour/article/view/763

Исследована антирадикальная активность (АРА) водорастворимых компонентов тканей 26 видов гидробионтов из семи классов: двустворчатые и головоногие моллюски, высшие раки, голотурии, морские звезды, морские ежи и лучеперые рыбы. Высокая антирадикальная активность (мг аск. к-ты/г) установлена для морской звезды патирии гребешковой (5,21); двустворчатых моллюсков: корбикула японская, мерценария Стимпсона, спизула сахалинская и глицемерис — соответственно 5,10; 1,15; 1,36 и 1,08; для гонад серого и черного морских ежей — соответственно 2,18 и 2,21. Достоверная корреляция была обнаружена между содержанием низкомолекулярных пептидов с молекулярной массой от 1 до 5 кДа и общей АРА (r = 0,801, r2 = 0,642).

Antiradical activity of water-soluble components of tissues is investigated for 26 species of marine organisms belonged to 7 classes: Bivalvia, Cephalopoda, Malacostraca, Holothuroidea, Asteroidea, Echinoidea, and Actinopterygii. Molecular weight of the proteins and peptides was measured in the water extracts using the modular liquid chromatograph (Agilent Technologies 1260 Infinity, USA) with UV detection at 280 nm in TSK gel column G 3000PWXL 7.8 mm I.D. × 30 cm (TOSOH Corporation, Japan) under flow rate of 0.3 mL/min and temperature of 25о C. All samples were twice prepared and double measured. The mobile phase consisted of 0.1 N NaCI 20 mM Tris-HCI buffer with pH 7.8. The following standard protein samples were used as a reference for the molecular weight measurement: bovine serum albumin (MW 66.3 kDa), egg albumin (44.3 kDa), myoglobin (18.0 kDa), cytochrome C (12.4 kDa), aprotinin (6.5 kDa), and bacitracin (1.4 kDa) (Sigma-Aldrich Co., USA). All samples were filtered before injection through 0.2 µm syringe filter (Whatman, PVDF). Molecular weight of peptides was calculated by the elution time. Scavenging effect on DPPH free radical was measured by Molyneux method and on ABTS radical – by the method proposed by Re et al. The maximum antiradical activity (measured by mg of ascorbic acid per 1 g) was found for the tissue of Patiria pectinifera (5.21), its various values were determined for the tissue of bivalve mollusks (Corbicula japonica, Mercenaria stimpsoni, Spisula sachalinensis, and Glycymeris yessoensis — 5.10; 1.15; 1.36 and 1.08, respectively) and medium values — for gonads of Strongylocentrotus intermedius and S. nudus (2.18 and 2.21, respectively). The amount of proteins and peptides with low molecular weight (1–5 kDa) correlated well with the radical scavenging activity (Pearson correlation coefficient 0.801, r2 = 0.642).

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The authors declare that there are no conflicts of interest present.