tinroИзвестия ТИНРОIzvestiya TINRO1606-99192658-5510ТИНРО10.26428/1606-9919-2022-202-946-956tinro-791Research ArticleТЕХНОЛОГИЯ ОБРАБОТКИ ГИДРОБИОНТОВTECHNOLOGY FOR PROCESSING OF AQUATIC ORGANISMS,Характеристика сульфатированных гликозаминогликанов хрящевой ткани скатов и осетровCharacteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeonhttps://orcid.org/0000-0002-5643-3601ЧепкасоваА. И.ChepkasovaA. I.

Чепкасова Анна Ивановна, кандидат технических наук, ведущий специалист

690091, г. Владивосток, пер. Шевченко, 4

Anna I. Chepkasova - Ph.D., leading specialist

690091, Vladivostok, Shevchenko Alley, 4

chepkasova@tinro-center.ruhttps://orcid.org/0000-0002-3228-3047СлуцкаяТ. Н.SlutskayaT. N.

Слуцкая Татьяна Ноевна, доктор технических наук, профессор, главный научный сотрудник

690091, г. Владивосток, пер. Шевченко, 4

Tatiana N. Slutskaya - D.Tech., professor, principal researcher

690091, Vladivostok, Shevchenko Alley, 4

slutskaya@tinro-center.ruhttps://orcid.org/0000-0001-8508-9603КарауловаЕ. П.KaraulovaE. P.

Караулова Екатерина Павловна, кандидат технических наук, ведущий научный сотрудник

690091, г. Владивосток, пер. Шевченко, 4

Ekaterina P. Karaulova - Ph.D., leading researcher

690091, Vladivostok, Shevchenko Alley, 4

karaulova2002@yandex.ruТихоокеанский филиал ВНИРО (ТИНРО)РоссияPacific branch of VNIRO (TINRO)Russian Federation2022311220222024946956Copyright © Чепкасова А.И., Слуцкая Т.Н., Караулова Е.П., 20232023Чепкасова А.И., Слуцкая Т.Н., Караулова Е.П.Chepkasova A.I., Slutskaya T.N., Karaulova E.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://izvestiya.tinro-center.ru/jour/article/view/791

Из хрящевой ткани ската и осетра (калуги) получены сульфатированные гликозаминогликаны (ГАГ) — хондроитинсульфаты — при следующих условиях: гидротермическая обработка сырья при 50 оС; гидролиз протеолитическим ферментом (гидромодуль 1 : 1; температура 45 оС; длительность 4 ч), последовательное осаждение ГАГ спиртом (2–3 объемами этилового спирта (96о) с высаливанием 2 %-ным раствором хлорида натрия. В полученных образцах определено содержание гексозаминов и сульфатированных ГАГ: установлено, что ГАГ из ската и калуги отличаются высоким содержанием сульфатированных форм, сравнимым с коммерческим препаратом, что открывает перспективы разработки технологии получения из отходов при разделке этих объектов монокомпонентных биологически активных добавок хондропротекторного действия.

Sulfated glycosaminoglycans (GAGs) or chondroitin sulfates were obtained from the cartilage tissue of skate (indefinite species) and sturgeon (kaluga) under the processes of hydrothermal treatment of raw materials at 50 оC, then hydrolysis with proteolytic enzyme in 4 hours under temperature 45 оC (hydromodulus 1 : 1), then sequential precipitation of GAGs in 2–3 volumes of 96о  ethyl alcohol and salting out with 2 % sodium chloride solution. The content of hexosamines and sulfated GAGs was determined in the extracted samples. GAGs of both skate and sturgeon have a high content of sulfated forms, comparable to commercial preparations, that opens up prospects for development of technology for production of monocomponent biologically active additives of chondroprotective action from the wastes of skates and sturgeons processing.

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The authors declare that there are no conflicts of interest present.