tinroИзвестия ТИНРОIzvestiya TINRO1606-99192658-5510ТИНРО10.26428/1606-9919-2023-203-214-222tinro-814Research ArticleУСЛОВИЯ ОБИТАНИЯ ПРОМЫСЛОВЫХ ОБЪЕКТОВENVIRONMENTS OF FISHERIES RESOURCESВлияние цинка и железа на динамику численности, морфологию и флуоресцентные характеристики микроводоросли Prorocentrum foraminosum (Dinophyta)Influence of zinc and iron on population dynamics, morphology and fluorescent properties of Prorocentrum foraminosum (Dinophyta)https://orcid.org/0000-0001-7135-1375МаркинаЖ. В.MarkinaZh. V.

Маркина Жанна Владимировна, кандидат биологических наук, научный сотрудник

690041, г. Владивосток, ул. Пальчевского, 17

Zhanna V. Markina, Ph. D., researcher

Vladivostok, 690041

zhannav@mail.ruhttps://orcid.org/0000-0002-9570-3192ОгнистаяА. В.OgnistayaA. V.

Огнистая Альбина Васильевна, аспирант

690041, г. Владивосток, ул. Пальчевского, 17;

690922, г. Владивосток, о. Русский, пос. Аякс, 10

Albina V. Ognistaya, graduate student

Vladivostok, 690041;

Ajax Bay, 10, Vladivostok, 690922

 

alya_lokshina@mail.ruНациональный научный центр морской биологии им. А.В. Жирмунского, ДВО РАНРоссияInstitute of Marine Biology. Far Eastern Branch. Russian Academy of Sciences. National Scientific Centre of Marine BiologyRussian FederationНациональный научный центр морской биологии им. А.В. Жирмунского, ДВО РАН; Дальневосточный федеральный университетРоссияInstitute of Marine Biology. Far Eastern Branch. Russian Academy of Sciences. National Scientific Centre of Marine Biology; Far Eastern Federal UniversityRussian Federation2023050420232031214222Copyright © Маркина Ж.В., Огнистая А.В., 20232023Маркина Ж.В., Огнистая А.В.Markina Z.V., Ognistaya A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://izvestiya.tinro-center.ru/jour/article/view/814

Дана оценка влияния тяжелых металлов цинка, железа в концентрациях 50 и 100 мкг/л на динамику численности, морфологию, флуоресценцию хлоропласта и зеленую автофлуоресценцию клеток (ЗАФ) микроводоросли <i>Prorocentrum foraminosum</i>. Оценка выполнена на третьи и седьмые сутки эксперимента. Оба элемента в концентрации, превышающей ПДК, снижают численность водоросли. Наибольшее влияние оказало железо. Однако изменения структур клеток при добавлении Fe3+ не наблюдалось. При добавлении Zn2+ выявлена деформация клеток и истечение клеточного содержимого P. foraminosum. Флуоресценция хлоропласта становилась менее интенсивной при воздействии Fe3+. ЗАФ при наличии в среде Zn2+ и Fe3+ увеличивалась.

Influence of heavy metals as zinc and iron with concentrations of 50 and 100 mg/L on dynamics of the cells abundance, morphology, fluorescence of chlorophyll a, and green autofluorescence is examined for the microalgae Prorocentrum foraminosum. The impact was measured on the 3rd and 7th days of the experiment. Both elements in concentration above the maximum permissible levels reduce the cells number, with the higher impact for Fe3+. However, Fe3+ caused no changes in structure of cells. The impact of Zn2+ caused the cells deformation and excretion of the cellular content. The chloroplast fluorescence became less intensive under influence of Fe3+, but the green autofluorescence had increased under presence of Zn2+ and Fe3+ in the medium.

: Prorocentrum foraminosumцинкжелезочисленность клетокфлуоресценция хлоропластазеленая автофлуоресценция динофлагеллят: Prorocentrum foraminosumzincironcell numberchloroplast fluorescencegreen autofluorescence of dinoflagellatesАвторы благодарят ресурсный центр «Морской биобанк» Национального научного центра им. А.В. Жирмунского за предоставление культуры Prorocentrum foraminosum MBRU_PrRUS_16. Результаты настоящего исследования были получены в рамках выполнения государственного задания Минобрнауки России «Динамика морских экосистем, адаптации морских организмов и сообществ к изменениям среды обитания», номер гос. регистрации ЕГИСУ 121082600038-3.The authors are grateful to the Marine Biobank Resource Center of the A.V. Zhirmunsky National Scientific Center for providing a culture of Prorocentrum foraminosum MBRU_PrRUS_16. 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The authors declare that there are no conflicts of interest present.