CONDITIONS FOR PRIMARY PRODUCTION OF PHYTOPLANKTON IN THE VOSTOK BAY (JAPAN SEA) IN SPRING 2016

The Vostok Bay was surveyed on March 16–18, 2016 with measuring of water properties profiles by oceanographic sondes Sea-Bird SBE-19plus V2 and Rinko Profiler ASTD-102 with sensors of pressure, temperature, conductivity, turbidity, chlorophyll fluorescence, dissolved oxygen, and photosynthetically active radiation (PAR) and collecting of water samples by SBE-32 carousel sampler with 10 liter bottles for further measuring of nutrients (P, Si and N in forms of nitrate and ammonium) and chlorophyll a concentration and phyto- and zooplankton abundance and species composition. Assimilation number (Pb ) of phytoplankton was determined using the optical sensor of dissolved oxygen mounted on logger Rinko AR01-USB and primary production was calculated from the measured values of Pb , Chl a and PAR. Values of primary production ranged from 200 to 2100 mgC/(m2.day). The highest phytoplankton growth was detected at the depth of 8–10 m in the northern Vostok Bay and 10–16 m in its southern part. The total daily production of phytoplankton within the Bay was estimated as 12.5 tC. Species composition of phytoplankton was formed mainly by diatoms (Bacillariophyta) and dinophytes (Dinophyta). The highest biomass of raw phytoplankton was registered at the sea surface, whereas the highest values of chlorophyll concentration occurred mainly at the bottom of the bay. Species composition of zooplankton was typical for spring season, with domination of copepods presented mainly by neritic species; its biomass was in 12 times lower than the phytoplankton biomass, on average. There was concluded that photosynthetic activity of phytoplankton was limited by nitrate availability, therefore it was intensified by penetration of relatively cold, nitrogen-rich waters from the deep-water sea to the Vostok Bay.

1. Gaiko, L.A., Marikul’tura: prognoz urozhaynosti s uchetom vozdeystviya abioticheskikh faktorov (Mariculture: forecast of productivity in view of influence of abiotic factors), Vladivostok: Dal’nauka, 2006.

2. Dultseva, O.A. and Odintsov, V.S., The number and activity of denitrifying bacteria in soft soils of the Vostok Bay of the Sea of Japan, Russ. J. Mar. Biol., 1994, vol. 20, no. 3, pp. 56–62.

3. Zvalinsky, V.I., Tishchenko, P.P., Tishchenko, P.Ya., Lobanov, V.B., Sagalaev, S.G., Shvetsova, M.G., Volkova, T.I., Sergeev, A.F., and Propp, L.N., Results of hydrochemical and production parameters survey in the Amur Bay water area during the Razdolnaya river flood in August 2005, in Sovremennoe sostoyanie i tendentsii izmeneniya prirodnoi sredy zaliva Petra Velikogo Yaponskogo morya (Current State and Trends in the Natural Environment of Peter the Great Bay, Sea of Japan), Moscow: GEOS, 2008, pp. 199–229.

4. Karedin, E.P., Instruktsiya po kolichestvennoy obrabotke morskogo setnogo planktona (Instructions for the quantitative processing of marine net plankton), Vladivostok: TINRO, 1982.

5. Kas’yan, V.V., Winter zooplankton in the water where the construction of Primorsky petrochemical plant, Universum: Chemistry and Biology: electron. scientific journals, 2014, no. 8(8). URL: http://7universum.com/ru/nature/archive/item/1514.

6. Kashenko, S.D., Soils of Vostok Bay, Sea of Japan, Biodiversity and Environment of Far East Reserves, 2014, no. 1, pp. 25–35.

7. Klimova, V.L., Assessment of the impact of soil dumping, based on biological parameters, at the dumping sites of the Sea of Japan, in Itogi issledovanii v svyazi so sbrosom otkhodov v more (Results of Investigations in Connection with Waste Dumping into Sea), Moscow: Gidrometeoizdat, 1988, pp. 137–141.

8. Konovalova, G.V., Seasonal characteristics of phytoplankton in the Amursky Bay of the Sea of Japan, Okeanol., 1972, vol. 12, no. 1, pp. 123–128.

9. Konovalova, G.V., Structure of plankton phytocenosis of the East Bay of the Sea of Japan, Sov. J. Mar. Biol., 1984, vol. 10, no. 1, pp. 13–23.

10. Mikulich, L.V. and Rodionov, N.A., Weight characteristics of some zooplankters of the Sea of Japan, Tr. Tikhookean. Okeanolog. Inst. Dal’nevost. Nauchn. Tsentra Akad. Nauk SSSR, 1975, no. 9, pp. 75–83.

11. Mokeeva, N.P., The response of marine biocenoses to ground dumping, in Itogi issledovanii v svyazi so sbrosom otkhodov v more (Results of Investigations in Connection with Waste Dumping into Sea), Moscow: Gidrometeoizdat, 1988, pp. 89–104.

12. Morozova, T.V. and Orlova, T.Yu., Monitoring of phytoplankton in the area of a sea farm in Vostok Bay (Sea of Japan), Russ. J. Mar. Biol., 2005, vol. 31, no. 1, pp. 1–6.

13. Ogorodnikova, A.A., Ekologo-ekonomicheskaya otsenka vozdeistviya beregovykh istochnikov zagryazneniya na prirodnuyu sredu i bioresursy zaliva Petra Velikogo (Ecological and Economic Assessment of the Impact of Coastal Pollution Sources on the Natural Environment and Bioresources of Peter the Great Bay), Vladivostok: TINRO-Tsentr, 2001.

14. Odum, E.P., Fundamentals of Ecology, Philadelphia ; L. ; Toronto : W.B Sounders, 1971.

15. Propp, M.V. and Propp, L.N., Hydrochemical basis of the process of primary production in the coastal region of the Sea of Japan, Sov. J. Mar. Biol., 1981, vol. 7, no. 1, pp. 29–37.

16. Selina, M.S., Phytoplankton Bay East of the Sea of Japan Extended Abstract of Cand. Sci. (Biol.) Dissertation, Vladivostok, 1998.

17. Stunzhas, P.A., Tishchenko, P.Ya., Ivin, V.V., Drummers, Yu.A., Volkova, T.I., Vyshkvartsev, D.I., Zvalinsky, V.I., Mikhailik, T.A., Semkin, P.Yu., Tishchenko, P.P., Khodorenko, N.D., Shvetsova, M.G., and Golovchenko, F.M., The first case of anoxia in the waters of the Far Eastern Marine Reserve, Dokl. Earth Sci., 2016, vol. 467, no. 2, pp. 218–221. doi 10.7868/S0869565216080211

18. Tishchenko, P.P., Zvalinsky, V.I., Tishchenko, P.Ya., and Semkin, P.Yu., The primary production of Amursky Bay (Sea of Japan) in the summer of 2008, Russ. J. Mar. Biol., 2017, vol. 43, no. 3, pp. 224–231.

19. Tishchenko, P.P., Tishchenko, P.Ya., Zvalinskii, V.I., and Sergeev, A.F., The carbonate system of Amur Bay (Sea of Japan) under conditions of hypoxia, Oceanology, 2011, vol. 51, no. 2, pp. 235–246.

20. Tishchenko, P.Ya., Mikhailik, T.A., Tishchenko, P.P., Shvetsova, M.G., Shkirnikova, E.M., Koltunov, A.M., Sergeev, A.F., and Zvalinskii, V.I., Peculiarities of hydrochemical characteristics of water of the Amur Bay in July 2008, Voda: Khim. Ekol., 2013, no. 9, pp. 3–10.

21. Tishchenko, P.Ya., Sergeev, A.F., Lobanov, V.B., Zvalinsky, V.I., Koltunov, A.M., Mikhailik, T.A., Tishchenko, P.P., and Shvetsova, M.G., Hypoxia of the bottom waters of Amursky Bay, Vestn. Dal’nevost. Otd. Ross. Akad. Nauk, 2008, no. 6, pp. 115–125.

22. Tkalin, A.V., Klimova, V.L., Shapovalov, E.N., Kulinich, N.M., Sevost’yanov, A.V., Belan, T.A., and Borisov, B.M., Some regional consequences of anthropogenic impacts on the marine environment, Tr. Dal’nevost. Nauchno–Issled. Gidrometeorol. Inst., Leningrad: Gidrometeoizdat, 1990, vol. 144.

23. Fedorov, V.D., O metodakh izucheniya fitoplanktona i ego aktivnosti (On the Methods for the Study of Phytoplankton and Its Activity), Moscow: Moscow Gos. Univ., 1979.

24. Khristoforova, N.K., Galysheva, Yu.A., and Kozhenkova, S.I., Assessment of human impact on Vostok Bay (Sea of Japan): Evidence from macrobenthic algae, Dokl. Earth Sci., 2005, vol. 405A, no. 9, pp. 1423–1425.

25. Chislenko, L.L., Nomogrammy dlya opredeleniya vesa vodnykh organizmov po razmeram i forme tela (Nomograms for determining the weight of aquatic organisms by size and body shape), Leningrad: Nauka, 1968.

26. Shkoldina, L.S., On the zooplankton of the marine reserve «Gulf of Vostok», in Mater. 5 Dal’nevost. konf. po zapovednomu delu (Materials of the V Far East. Conf. on Conservation Matter), Vladivostok: Dal’nauka, 2001, pp. 317–318.

27. Battye, W., Aneja, V.P., and Schlesinger, W.H., Is nitrogen the next carbon?, Earth’s Future, 2017, vol. 5, no. 9, pp. 894–904. doi 10.1002/2017EF000592

28. Breitburg, D., Levin, L.A., Oschlies, A., Grégoire, M., Chavez, F.P., Conley, D.J., Garçon, V., Gilbert, D., Gutiérrez, D., Isensee, K., Jacinto, G.S., Limburg, K.E., Montes, I., Naqvi, S.W.A., Pitcher, G.C., Rabalais, N.N., Roman, M.R., Rose, K.A., Seibel, B.A., Telszewski, M., Yasuhara, M., and Zhang, J., Declining oxygen in the global ocean and coastal waters, Science, 2018, vol. 359, no. 6371, pp. 1–11. doi 10.1126 / science.aam7240

29. Cai, W.-J., Hu, X., Huang, W.-J., Murrell, M.C., Lehrter, J.C., Lohrenz, S.E., Chou, W.-C., Zhai, W., Hollibaugh, J.T., Wang, Y., Zhao, P., Guo, X., Gundersen, K., Dai, M., and Gong, G.-C., Acidification of subsurface coastal waters enhanced by eutrophication, Nature Geoscience, 2011, vol. 4, no. 11, pp. 766–770. doi 10.1038/NGEO1297

30. Calliari, D., Short-term changes in the concentration and vertical distribution of chlorophyll and in the structure of the microplankton assemblage due to a storm, Pan Am. J. Aquat. Sci., 2007, vol. 2, no. 1, pp. 13–22.

31. Cullen, J.J. and Eppley, R.W., Chlorophyll maximum layers of the Southern California Bight and possible mechanisms of their formation and maintenance, Oceanol. Acta, 1981, vol. 4, no. 1, pp. 23–32.

32. Cullen, J.J., Yang, X., and MacIntyre, H.L., Nutrient Limitation of Marine Photosynthesis, in Primary Productivity and Biogeochemical Cycles in the Sea, New York: Plenum Press, 1992, pp. 69–88.

33. Doney, S.C., The Growing Human Footprint on Coastal and Open-Ocean Biogeochemistry, Science, 2010, vol. 328, no. 5985, pp. 1512–1516.

34. Franks, P.J.S. and Walstad, L.J., Phytoplankton patches at fronts: A model of formation and response to wind events, J. Mar. Res., 1997, vol. 55, no. 1, pp. l–29. doi 10,1357/0022240973224472

35. Gordon, H.R. and McCluney, W.R., Estimation of the depth of sunlight penetration in the sea for remote sensing, Appl. Opt., 1975, vol. 14, no. 2, pp. 413–416. doi 10.1364/AO.14.000413

36. Jeffrey, S.W. and Humphrey, G.F., New Spectrophotometric Equations for Determining Chlorophylls a, b, c1 and c2 in Higher Plants, Algae and Natural Phytoplankton, Biochem. Physiol. Pflanzen, 1975, vol. 167, no. 2, pp. 191–194. doi 10.1016/S0015-3796(17)30778-3

37. Kruskopf M. and Flynn K.J. Chlorophyll content and fluorescence responses cannot be used to gauge reliably phytoplankton biomass, nutrient status of growth rate, New Phytologist, 2006, vol. 169, no. 3, pp. 525–536. doi 10.1111/j.1469-8137.2005.01601.x

38. Laws, E.A., Photosynthetic quotients, new production and net community production in the open ocean, Deep-Sea Res. I, 1991, vol. 38, no. 1, pp. 143–167. doi 10.1016/0198-0149(91)90059-O

39. Rabalais, N.N., Cai, W.-J., Carstensen, J., Conley, D.J., Fry, B., Hu, X., Quiñones-Rivera, Z., Rosenberg, R., Slomp, C.P., Turner, R.E., Voss, M., Wissel, B., and Zhang, J., Eutrophicationdriven deoxygenation in the coastal ocean, Oceanography, 2014, vol. 27, no. 1, pp. 172–183. doi 10.5670/oceanog.2014.21

40. Rabalais, N.N., Turner, R.E., Díaz, R.J., and Justić, D., Global change and eutrophication of coastal waters, ICES J. Mar. Sci., 2009, vol. 66, no. 7, pp. 1528–1537. doi 10.1093/icesjms/fsp047

41. Redfield, A.C., The biological control of chemical factors in the environment, Am. Sci., 1958, vol. 46, pp. 205–221.

42. Ryther, J.H., The measurement of primary production, Limnology and Oceanography, 1956, vol. 1, no. 2, pp. 72–84. doi 10.4319/lo.1956.1.2.0072

43. Selman, M., Greenhalgh, S., Diaz, R., and Sugg, Z., Eutrophication and Hypoxia in Coastal Areas: A Global Assessment of the State of Knowledge, WRI Policy Note Water Quality: Eutrophication and Hypoxia № 1, Washington, DC: World Resources Institute, 2008. https://www.wri.org/ publication/eutrophication-and-hypoxia-coastal-areas

44. Smith, L.M., Silver, C.M., and Oviatt, C.A., Quantifying variation in water column photosynthetic quotient with changing field conditions in Narragansett Bay, RI, USA, J. Plankton Res., 2012, vol. 34, no. 5, pp. 437–442. doi 10.1093/plankt/fbs011

45. Tishchenko, P., Tishchenko, P., Lobanov, V., Sergeev, A., Semkin, P., and Zvalinsky, V., Summertime in situ monitoring of oxygen depletion in Amursky Bay (Japan/East Sea), Cont. Shelf Res., 2016, vol. 118, pp. 77–87. doi 10.1016/j.csr.2016.02.014

46. Lotsiya severo-zapadnogo berega Yaponskogo morya. Ot reki Tumannaya do mysa Belkina (Northwestern Sea of Japan Coast Pilot. From the Tumen River to Cape Belkin), St. Petersburg: Gl. Upr. Navig. Okeanogr. Minist. Oborony, 1996, no. 1401.

47. Metody gidrokhimicheskikh issledovanii osnovnykh biogennykh elementov (Methods of Hydrochemical Studies of Main Nutrients), Moscow: VNIRO, 1988.