FRACTIONAL COMPOSITION OF WATER-SOLUBLE PROTEIN COMPONENTS OF DRY FERMENTOLIZATE AND STARTER FEED FOR SALMON FISH
https://doi.org/10.26428/1606-9919-2019-199-241-248
Abstract
Data on fractional composition of proteins and peptides in the dry fermentolizate and starter feed developed in TINRO are presented and compared with that composition in natural prey consumed by salmon juveniles and in compound feed for them used in fish farms. The dry fermentolizate is produced on base of raw pollock with successive processes of grinding, fermentation (45 min. under temperature 45–50 о C with complex enzyme preparation Protosubtilin G3x), bone separation, and pasteurization (15–20 min. under temperature 85–90 оC). The starter feed includes fish flour (pollock), wheat flour, dry fermentolizate, bran, hydrolysis yeast, milk powder, premix, and vegetable oil in a certain proportion. Methods of fractional composition investigation for the water-soluble part of protein component in feeding products are described in detail. The fractional composition is determined using the medium pressure gel permeation chromatography. General chemical composition of the high-protein dry fermentolizate and starter feed is presented. Contents of polypeptides and low molecular weight proteins with MM of 1.0–10.0 kDa, proteins with MM of 0.1–10.0 kDa and > 10.0 kDa for the new-developed starter feed with addition of dry fermentolizate coincide with those for natural prey of salmon larvae and juveniles (chironomids, oligochaetes, gammarus), so it corresponds to real nutritional needs of juvenile salmon.
About the Authors
A. N. Bashtovoy
Pacific branch of VNIRO (TINRO)
Russian Federation
Bashtovoy Alexander N., Ph.D., head of sector
Russian Federation
Bashtovoy Alexander N., Ph.D., head of sector
E. P. Karaulova
Pacific branch of VNIRO (TINRO)
Russian Federation
Karaulova Ekaterina P., Ph.D., leading researcher
Russian Federation
Karaulova Ekaterina P., Ph.D., leading researcher
G. N. Timchishina
Pacific branch of VNIRO (TINRO)
Russian Federation
Timchishina Galina N., Ph.D., associate professor, leading researcher
Russian Federation
Timchishina Galina N., Ph.D., associate professor, leading researcher
K. G. Pavel
Pacific branch of VNIRO (TINRO)
Russian Federation
Pavel Konstantin G., Ph.D., leading specialist
Russian Federation
Pavel Konstantin G., Ph.D., leading specialist
P. L. Pasechnik
Primorsky Branch of Glavrybvod
Russian Federation
Pasechnik Pavel L., deputy head
Russian Federation
Pasechnik Pavel L., deputy head
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Review
For citations:
Bashtovoy A.N., Karaulova E.P., Timchishina G.N., Pavel K.G., Pasechnik P.L. FRACTIONAL COMPOSITION OF WATER-SOLUBLE PROTEIN COMPONENTS OF DRY FERMENTOLIZATE AND STARTER FEED FOR SALMON FISH. Izvestiya TINRO. 2019;199(4):241-248. (In Russ.) https://doi.org/10.26428/1606-9919-2019-199-241-248
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