Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon
https://doi.org/10.26428/1606-9919-2022-202-946-956
Abstract
Sulfated glycosaminoglycans (GAGs) or chondroitin sulfates were obtained from the cartilage tissue of skate (indefinite species) and sturgeon (kaluga) under the processes of hydrothermal treatment of raw materials at 50 оC, then hydrolysis with proteolytic enzyme in 4 hours under temperature 45 оC (hydromodulus 1 : 1), then sequential precipitation of GAGs in 2–3 volumes of 96о ethyl alcohol and salting out with 2 % sodium chloride solution. The content of hexosamines and sulfated GAGs was determined in the extracted samples. GAGs of both skate and sturgeon have a high content of sulfated forms, comparable to commercial preparations, that opens up prospects for development of technology for production of monocomponent biologically active additives of chondroprotective action from the wastes of skates and sturgeons processing.
Keywords
skate,
kaluga,
cartilage tissue,
chondroitin sulfate,
glycosaminoglycans,
sulfated glycosaminoglycans,
hexosamines,
chondroprotector
Supporting agencies: The authors are deeply grateful to the staff of Luchegorsk research station of TINRO for the samples of kaluga tissues for the investigation.
About the Authors
A. I. Chepkasova
Pacific branch of VNIRO (TINRO)
Russian Federation
Russian Federation
Anna I. Chepkasova - Ph.D., leading specialist
690091, Vladivostok, Shevchenko Alley, 4
T. N. Slutskaya
Pacific branch of VNIRO (TINRO)
Russian Federation
Russian Federation
Tatiana N. Slutskaya - D.Tech., professor, principal researcher
690091, Vladivostok, Shevchenko Alley, 4
E. P. Karaulova
Pacific branch of VNIRO (TINRO)
Russian Federation
Russian Federation
Ekaterina P. Karaulova - Ph.D., leading researcher
690091, Vladivostok, Shevchenko Alley, 4
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For citations:
Chepkasova A.I., Slutskaya T.N., Karaulova E.P. Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon. Izvestiya TINRO. 2022;202(4):946-956. (In Russ.) https://doi.org/10.26428/1606-9919-2022-202-946-956
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